Testing of efficacy against viruses according to European standard EN 14476 The practical test (in-vitro test). This examines how the disinfectant acts on the test pathogens with the addition of an organic load (e.g. protein, blood). ... 1 ml of the mixture is used for further analysis. In order to be effective within a certain spectrum of activity, the disinfectant must inactivate a certain number of test viruses. Test methods of disinfectants: Viruses Level 1 virucidal activity against enveloped viruses All enveloped viruses spectrum of efficacy Test pathogens: • Vacciniavirus Level 3 virucidal All enveloped + all non-enveloped viruses spectrum of efficacy Test pathogens: • Poliovirus • murine Norovirus • Adenovirus • murine Parvovirus (only for application >40 °C) After a defined time ... There are specific test pathogens for each spectrum of activity. They are mixed together with an organic load (e.g. blood) and the disinfectant used for testing. Level 2 limited spectrum virucidal activity All enveloped + 3 non-enveloped viruses (Adeno-, Rota- and Noroviruses) spectrum of efficacy Test pathogens: • murine Norovirus • Adenovirus 3 HSC EN 02.22
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